In the original article Zhu et al. (2017) was not cited in the article. The citation has now been inserted in Materials and Methods, Plasmids Constructions, Paragraph 1 and Table 1 and should read:
Table 1.
Strains and plasmids used in this study.
| Strains and plasmids | Description | Reference or source |
|---|---|---|
| STRAINS | ||
| C. hutchinsonii | Wild type | ATCC |
| ATCC 33406 | ||
| Δ3195 | Targeted insertion in chu_3195; Emr | This study |
| Δ3196 | Targeted insertion in chu_3196; Emr | This study |
| Δ3198 | Targeted deletion of chu_3198; Emr | This study |
| Δ3199 | Targeted insertion in chu_3199; Emr | This study |
| Δ1253 | Targeted insertion in chu_1253; Emr | This study |
| COM3195 | Complemented strain with plasmid pCH3195; Emr, Cmr | This study |
| COM3199 | Complemented strain with plasmid pCH3199; Emr, Cmr | This study |
| E. coli DH5α | F −φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rk−, mk+) phoA supE44 λ − thi-1 gyrA96 relA1 | Laboratory stock |
| PLASMIDSa | ||
| pLYL03 | ColE1; Bacteroides–Flavobacterium suicide vector; Apr (Emr) | Li et al., 1995 |
| pYT313 | sacB-containing suicide vector; Apr (Emr) | Zhu et al., 2017 |
| pLYIN3195 | pLYL03 carrying an 1.0-kbp internal fragment of chu_1719; Apr (Emr) | This study |
| pYT3198 | PYT313 carrying two 2.0-kbp fragments upstream and downstream of chu_3198; Apr (Emr) | This study |
| pLYIN3199 | pLYL03 carrying an 748-bp internal fragment of chu_3196; Apr (Emr) | This study |
| pLYIN1253 | pLYL03 carrying an 825-bp internal fragment of chu_1253; Apr (Emr) | This study |
| pCH03C | pLYL03oriC containing cat resistant gene; Apr (Emr, Cmr) | Zhou et al., 2016 |
| pCH3195 | pCH03C containing an expression cassette of chu_3195 under control of the chu_1284 promoter; Apr (Emr, Cmr) | This study |
| pCH3198 | pCH03C containing an expression cassette of chu_3198 under control of the PompA promoter; Apr (Emr, Cmr) | This study |
| pCH3199 | pCH03C containing an expression cassette of chu_3199 under control of the chu_1284 promoter; Apr (Emr, Cmr) | This study |
Antibiotic resistance phenotypes: ampicillin (Apr), chloramphenicol (Cmr), erythromycin (Emr), kanamycin (Kmr). Unless indicated otherwise, the antibiotic resistance phenotypes are those expressed in E. coli. The antibiotic resistance phenotypes in parentheses are expressed in C. hutchinsonii.
To generate the pYT3198 plasmid, two DNA fragments corresponding to approximately 2 kb of chu_3198 up- and downstream regions were amplified from C. hutchinsonii chromosomal DNA with the primers 3198upF/3198upR, and 3198downF/3198downR, respectively, and ligated into the pYT313 plasmid (Zhu et al., 2017).
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way.
Conflict of interest statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
References
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