Figure 6. Demonstrative raw signal for a non-chimeric read (from an Ifnb1 amplicon).

The recorded signal for this read starts with a very long period of 7s in the open pore state, followed by a short stall of 0.3s, then a coding Ifnb1 sequence that took 2.5s to transition through the pore, then an NB05-flanked non-coding Ifnb1 sequence that took 2s seconds to transition through the pore. Note: These figures have been annotated with approximate region boundaries based on the order of hits to the base-called sequence.