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. 2017 Apr 11;8(35):58480–58493. doi: 10.18632/oncotarget.17024

Table 1. Primer sequences used in real-time quantitative PCR analysis.

Type Name Primer Annealing temperature(°C)
Type Sequence, 5’-3’
Mice IL-8 Sense ACA TGA CTT CCA AGC TGG CCG T 52
Antisense CCT CTT CAA AAA CTT CTC CAC AAC
M-CSF Sense AGC AGG AGT ATC ACC GAG GA 52
Antisense TAT CTC TGA AGC GCA TGG TG
PTHrP Sense ATG CAG CGG AGA CTG GTT CAG 58
Antisense TTC TAG TGC CAC TGC CCA TTG
OPG Sense CTT CGT GCC TTG ATG GA 44
Antisense TTG GGA AAG TGG GAT GT
RANKL Sense ACC AAG ATG GCT TCT ATT ACC 44
Antisense TCC CTC CTT TCA TCA GGT TAT
GAPDH Sense GGT CGG AGT CAA CGG ATT TG 58
Antisense ATG AGC CCC AGC CTT CTC CAT
Cell TGF-β1 Sense AGC GAC TCG CCA GAG TGG TTA 58
Antisense GCA GTG TGT TAT CCC TGC TGT CA
Smad 2 Sense TTA ACC GAA ATG CCA CGG TAG AA 60
Antisense GCT CTG GAC AAA CAT TGC ACT ATC A
Smad 3 Sense AGG CGT GCG GCT CTA CTA CAT C 60
Antisense CAG CGA ACT CCT GGT TGT TGA A
Smad 4 Sense CAG CAC TAC CAC CTG GAC TGG A 60
Antisense CTG GAA TGC AAG CTC ATT GTG AA
Smad 7 Sense TGC TGT GCA AAG TGT TCA GGT G 60
Antisense CCA TCG GGT ATC TGG AGT AAG GA
GAPDH Sense GCA CCG TCA AGG CTG AGA AC 58
Antisense TGG TGA AGA CGC CAG TGG A