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. 2017 Aug 15;22(8):1355. doi: 10.3390/molecules22081355

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Comparison of the previous [15] and current pulse sequences for measuring ¹⁵N relaxation of NH₃⁺ groups. (a,b) ¹⁵N longitudinal (Panel a) and transverse (Panel b) relaxation of the K46 NH₃⁺ group. The vertical axis represents the signal intensity in the two-dimensional spectra measured as a function of the relaxation period T_r. Solid lines represent the best-fit curves obtained through nonlinear least-squares fitting with a mono-exponential function; (c) Slices of the K46 NH₃⁺ signals along the ¹H dimension from the two-dimensional spectra with and without ¹H saturation for the heteronuclear NOE measurements. In each panel, data obtained with the previous and current pulse sequences are shown in blue and red, respectively.