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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1999 Mar 2;96(5):2569.
PMCID: PMC56035

Cell Biology. In the article “ADP-ribosylation factor and phosphatidic acid levels in Golgi membranes during budding of coatomer-coated vesicles” by Mark Stamnes, Giampietro Schiavo, Gudrun Stenbeck, Thomas H. Söllner, and James E. Rothman, which appeared in number 23, November 10, 1998, of Proc. Natl. Acad. Sci. USA (95, 13676–13680), the authors wish to note the following correction. The label, described correctly in the legend to Fig. 1, incorrectly indicated that coatomer was included in stage 1 of the two-stage reactions. A corrected figure and its legend are shown below.

Figure 1.

Figure 1

ARF and coatomer binding in one- and two-stage reactions. The amount of membrane-bound ARF and coatomer (β-COP) determined by Western blot analysis of binding reactions. Lanes 1 and 2 show one-stage reactions in which ARF and coatomer were incubated together. Lanes 3–6 show the results from two-stage reactions in which the membranes were first incubated with ARF but not coatomer, reisolated, and then incubated in a second stage with coatomer but not ARF. As controls, membranes (lane 2) or ARF (lane 3) were excluded from stage 1, or coatomer (lane 4) was excluded from both stages. All incubations were carried out at 37°C except lane 5, which was carried out at 0°C.


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