Correction to Biochemical and Spectroscopic Characterization of the Non-Heme Fe(II)- and 2-Oxoglutarate-Dependent Ethylene-Forming Enzyme from Pseudomonas syringae pv. phaseolicola PK2

An error in protein concentration determination resulted in specific activity, k_cat, k_cat/K_m, and extinction coefficient values that were 4-fold too large.

Page 5993. The specific activity should read 548 ± 30 units/mg, which is comparable to the previously reported value of 660 units/mg. The k_cat values for 2OG, L-Arg (measuring ethylene), and L-Arg (measuring P5C) in Table 1 and the text should read 31 ± 3, 32 ± 1, and 0.73 ± 0.08 min⁻¹, respectively. The corresponding k_cat/K_m values should read 0.55, 0.87, and 0.015 μM⁻¹ min⁻¹, respectively.

Pages 5994 and 5995. The kcat and k_cat/K_m values for 2OA in Table 2 and the text should read 0.063 ± 0.005 min⁻¹ and 0.002 μM⁻¹ min⁻¹, respectively, and those for L-Arg should be 0.068 ± 0.003 min⁻¹ and 0.001 μM⁻¹ min⁻¹, respectively. The extinction coefficient at 515 nm for EFE/Fe(II)/2OG should read ~28 M⁻¹ cm⁻¹, and that for EFE/Fe(II)/2OG/L-Arg should read ~79 M⁻¹ cm⁻¹.

Page 5996. The extinction coefficient at 515 nm for EFE/Fe(II)/2OA should read ~26 M⁻¹ cm⁻¹, and that for EFE/Fe(II)/2OA/L-Arg should read ~39 M⁻¹ cm⁻¹. In Figures S2–S4 of the Supporting Information, the y-axes should be multiplied by 0.25.

These changes do not affect the conclusions of the paper regarding the dual-circuit mechanism of the ethylene-forming enzyme.