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. 2017 Sep 19;6:e30647. doi: 10.7554/eLife.30647

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© 2017, Andrews et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 1. — Brachial (A, B, E, F, G, H, I, J, K, L, M, N, Q, U, X, Y, BB) or thoracic (C, D, O, P, R, S, T, V, W, Z, AA) level transverse sections from Hamburger-Hamilton (HH) stage 14–24 chicken spinal cords processed for immunohistochemistry (A–D, I-–L, Q–T) or in situ hybridization (E–H, M–P, U–BB). (A–D) Pax3 is present in dorsal progenitors prior to HH stage 14 and persists throughout dorsal spinal cord patterning and differentiation. (E–H) Atoh1 expression in dP1 progenitors begins prior to HH stage 18. (I–L) Lhx2/9⁺ dI1 neurons start to be born at the brachial-most levels of HH stage 18 embryos. (M–P) Ascl1 expression in dP3-5 progenitors begins prior to HH stage 16. (Q–T) Isl1⁺ dI3 neurons are born starting from HH stage 21. (U–X) Bmp4 is expressed in cells flanking the RP prior to HH stage 17 and stays tightly localized to this region in the dorsal spinal cord. (Y–BB) Bmp7 expression starts at ~HH stage 14, and it is expressed more broadly in the dorsal-most spinal cord, including the RP. (CC)A timeline summarizing the onset of Bmp expression and generation of dI1 and dI3 neurons. Scale bar: 50 µm.

Figure 1—figure supplement 1. — Brachial (A, B, E, F, G, H, I, J, K, L, M, N, Q, U, X, Y, BB) or thoracic (C, D, O, P, R, S, T, V, W, Z, AA) level transverse sections from Hamburger-Hamilton (HH) stage 14–24 chicken spinal cords processed for immunohistochemistry (A–D, I-–L, Q–T) or in situ hybridization (E–H, M–P, U–BB). (A–D) Pax3 is present in dorsal progenitors prior to HH stage 14 and persists throughout dorsal spinal cord patterning and differentiation. (E–H) Atoh1 expression in dP1 progenitors begins prior to HH stage 18. (I–L) Lhx2/9⁺ dI1 neurons start to be born at the brachial-most levels of HH stage 18 embryos. (M–P) Ascl1 expression in dP3-5 progenitors begins prior to HH stage 16. (Q–T) Isl1⁺ dI3 neurons are born starting from HH stage 21. (U–X) Bmp4 is expressed in cells flanking the RP prior to HH stage 17 and stays tightly localized to this region in the dorsal spinal cord. (Y–BB) Bmp7 expression starts at ~HH stage 14, and it is expressed more broadly in the dorsal-most spinal cord, including the RP. (CC)A timeline summarizing the onset of Bmp expression and generation of dI1 and dI3 neurons. Scale bar: 50 µm.