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. Author manuscript; available in PMC: 2018 Sep 20.
Published in final edited form as: Metallomics. 2017 Sep 20;9(9):1260–1270. doi: 10.1039/c7mt00104e

Figure 2. TD-1 effects on the kinetics of deoxyHbS polymer formation.

Figure 2

(A) Concentration dependent aggregation of deoxyHbS in 1.8 M phosphate buffer. Solutions of deoxyHb in a range of concentrations (80 μM, 150 μM, 300 μM) were rapidly heated from 0°C to 30°C. Turbidity was measured spectrophotometrically by recording the absorbance at 700 nm. (B) Polymerization of deoxyHbS solution (150 μM) in the presence and absence of TD-1 (450 μM) were measured from 0°C to 30°C. Turbidity was measured spectrophotometrically by recording the absorbance at 700 nm.