Fig. 2.

RAN action on proliferative myoblasts: 24 h. To investigate whether RAN could enhance myogenic phenotype acquisition, we analyzed myoblasts morphology and Myf5, p21 and MyoD protein expression. Immunofluorescence images and quantification (20X): RAN increased the signal of Myf5, p21 and MyoD proteins in respect of GM and DM at 24 h. These images showed the important morphological changes and the different localization of the signal in myoblasts treated with RAN with respect to GM and DM control. Objective: 20X. t test: *p ≤ 0.05 or **0.01 vs. GM