Figure 1. The levels of circulating miR-24.

Expression of miR-24 mRNA level was confirmed through qRT-PCR analysis in control, CHD, and DM2-CHD, respectively. The products of qRT-PCR were detected by agarose gel electrophoresis. Total RNA extraction was performed using TRIzol Reagent. The data were normalized to U6 RNA in each sample. The qRT-PCR data were analyzed using the 2 ^−ΔΔCt method. *P<0.05, Control vs CHD, DM2-CHD; ^# P<0.05 vs CHD group.