Figure 7. Binding activity of Y498 with the wild-type (WT) and mutant HIV-1 Envs determined by IFA.

The plasmids encoding Envs were transiently expressed in 293T cells by transfection. Y498 was tested at a concentration of 20μg/ml and detected by a DyLight®488 labeled-rabbit anti-human antibody. The experiments were repeated three times and the representative data are shown. The amino acid numbering of Env is based on the HIV-1 HXB2 sequence.