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. 2017 Jun 29;8(38):63646–63664. doi: 10.18632/oncotarget.18882

Figure 3. Relationship between LEFTY expression and TGF-β1/Smad signaling.

Figure 3

(A) Western blot analysis of LEFTY, pSmad2, and Smad2 in OVISE, TOV-21G, and Ishikawa cells treated with 2 ng/mL TGF-β1 for 0, 12, and 24 hours. (B) Ishikawa and TOV-21G cells were transfected with LEFTY1 and LEFTY2 reporter constructs, together with Smad2 and TGF-β1 treatment. Relative activity was determined based on arbitrary light units of luciferase activity normalized to pRL-TK activity. The activities of the reporter plus the effector relative to that of the reporter plus empty vector are shown as mean±SD. The experiment was performed in duplicate. (C) HE staining and IHC for LEFTY and pSmad2 in semi-serial sections of OCCCa. Note the pSmad2 immunoreactivity in both nuclear and cytoplasmic compartments. Original magnification, x200. (D) Correlation between LEFTY and pSmad2 scores in OCCCa.