Table 2. Primer sequences used in this study.

Gene	Assay		Sequence
LEFTY1	Promoter	Forward	5′-TGCTAGGCACCCTGTAGACA-3′
		Reverse	5′-AAGGCTGCAGGAGGGTCTCA-3′
	mRNA	Forward	5′-CTGCTGATGGACAAATGCTCTG-3′
	/ISH probe	Reverse	5′-ACTTTAGCCCAGATCCAGTGAC-3′
	Methylation	Forward	5′-TAGTTTTTAAGGTTTAGGGTGTG-3′
		Reverse	5′-TACTAACCCTACTCTTATCCC-3′
	sh 1207-1229	Forward	5′-GATCCCGACTTGTGTGTGTTTCTGAAGGCTTCC
			TGTCACCTTCAGAAACACACACAAGTCTTTTTTG-3′
		Reverse	5′-AATTCAAAAAAGACTTGTGTGTGTTTCTGAAGG
			TGACAGGAAGCCTTCAGAAACACACACAAGTCGG-3′
LEFTY2	Promoter	Forward	5′-TTCCCTGTTCTTCAAACACCGTCC-3′
		Reverse	5′-TCCTCTAGGGAGGTTGAAGGAGG-3′
	mRNA	Forward	5′-TCTAACTGAACGTGTGCATAG-3′
	/ISH probe	Reverse	5′-GAAGAATTTGCCAGAGAACAG-3′
XIAP	mRNA	Forward	5′-GCTAACTGATTGGAAGCCC-3′
		Reverse	5′-GTCCTTGAAACTGAACCCC-3′
	Promoter	Forward	5′-CTAGGTCAGCTTCTCGGTTCCAG-3′
		Reverse	5′-GAGAAACCCGGAGTCGTGAAACC-3′

ISH, in situ hybridization; sh, short-hairpin.