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. 2017 Jul 31;8(38):64001–64014. doi: 10.18632/oncotarget.19699

Figure 1. ZEA exposure increased cell apoptosis and induced the apoptosis-related gene mRNA abundance in cultured granulosa cells.

Figure 1

(A) Cultured granulosa cells in vitro. (B) Immunofluorescent staining of TUNEL and DAPI. Bars indicate 50 μm. (C) Graph indicating the percentages of TUNEL positive granulosa cells. (D) The mRNA levels of Bax/Bcl-2 in cultured granulosa cells exposed to ZEA and control groups as determined by Q-PCR. The results are presented as mean ± SD. All experiments were repeated at least three times. * P < 0.05; ** P < 0.01.