Skip to main content
. Author manuscript; available in PMC: 2018 Sep 21.
Published in final edited form as: Cell. 2017 Sep 21;171(1):133–147.e14. doi: 10.1016/j.cell.2017.08.044

Figure 1. We Developed a High-Throughput Pipeline to Determine the Localization and Physical Interactions of Algal Proteins.

Figure 1

(A) A false-color transmission electron micrograph of a Chlamydomonas reinhardtii cell. The chloroplast is highlighted in magenta and the pyrenoid matrix in orange.

(B) Tagging and mass spectrometry pipeline. Target genes were amplified by PCR and Gibson assembled in frame with Venus-3xFLAG, under the constitutive PSAD promoter. Transformants were screened for fluorescence using a scanner, and arrayed to allow robotic propagation. Lines were either imaged using confocal microscopy to determine their spatial distribution or batch cultured for affinity purification-mass spectrometry (AP-MS).