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. 2017 Jun 1;8(37):60826–60840. doi: 10.18632/oncotarget.18338

Figure 5. Chemoattractive effect of IGFBP-6 on monocytes, T lymphocytes and B lymphocytes.

Figure 5

A., B., C. Monocytes (A), T lymphocytes (B) and B lymphocytes (C) were placed in the upper compartment of a Transwell in the absence or presence of IGFBP-6 at different concentrations in the lower compartment. Number of migrating cells across a 3 µm-pore membrane after 150 min was measured by flow cytometry. As a positive control, experiments were conducted in the presence of different concentrations of SDF-1 in the lower compartment. Results are shown as mean ±SEM of three independent experiments and expressed as a percentage of controls obtained in the absence of IGFBP-6 in the basolateral compartment and set at 100%. *p < 0.05; **p < 0.001; ***p < 0.0001. D., E., F. In order to assess the specificity of the IGFBP-6 chemoattractive effect, monocytes (D), T lymphocytes (E) and B lymphocytes (F) were placed in the upper compartment of a Transwell in the presence in the lower compartment of: IGFBP-6 (40 nM); IGFBP-6 (40 nM) pre-incubated with an antibody directed against IGFBP-6; IGFBP-6 (40 nM) pre-incubated with an irrelevant antibody; Activin A (40 nM) as negative control. Results are shown as mean ±SEM of three independent experiments and expressed as a percentage of control. *p < 0.05; **p < 0.001. Results were analyzed using 2-way ANOVA with Tukey’s Multiple Comparison test.