Figure 1. Characterization of a RAS-transformed human fimbria epithelial cell line.

(A) Human fimbria epithelial cells (FTE) were primarily cultured, transduced with pLenti-E6/E7 and pLenti-hTERT. The immortalized cell line FE25 was derived. By additional transduction with pLenti-Ras^V12 oncogene, FE-RAS cells were derived. Western blot results showed the expression pattern of RAS, PAX8, p53, HPV E6 and E7 in FE25 and FE-RAS cells. (B) Immunohistochemistry of xenotumors from FE-RAS cells subcutaneous (upper panel) and intraperitoneal (lower panel) injections into NSG mice. Scale bars: 50 μm.