Table 1. Primers used in the study.
| Primer name | Sequence | Purpose | |
|---|---|---|---|
| A | KIFC1XYX-F2 | AGAAACCCAGCAAACGTCCA | Semi-quantitative RT-PCR for endogenous kifc1 |
| B | KIFC1XYX-R2 | AGTTGGGACATCAGTCCCCT | Semi-quantitative RT-PCR for endogenous kifc1 |
| C | GADPH-F | ACCACAGTCCATGCCATCAC | Internal control of semi-quantitative RT-PCR |
| D | GADPH-R | TCCACCACCCTGTTGCTGTA | Internal control of semi-quantitative RT-PCR |
| E | KIFC1KC-XYX-F1 | CCGCTCGAGATGGATCCGCAGAGGTCCCC | Construction of ΔC-pCMV-N-FLAG plasmid |
| F | KIFC1KC-XYX-B1 | TGCTCTAGAGCCCTTGAGTTCCTGCAGCTG | Construction of ΔC-pCMV-N-FLAG plasmid |
| G | M13F | CGCCAGGGTTTTCCCAGTCACGAC | Colony PCR when constructing the Δ C-pCMV-N-FLAG plasmid |
| H | M13R | AGCGGATAACAATTTCACACAGGA | Colony PCR when constructing the Δ C-pCMV-N-FLAG plasmid |