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. 2017 Jun 27;8(37):61674–61686. doi: 10.18632/oncotarget.18669

Figure 3. Knockdown of IGFBP-2 attenuates EMT through the Hedgehog pathway by suppressing PTCH1.

Figure 3

(A) Relative expressions of IGFBP-2, PTCH1 and Gli1 were evaluated by Western blotting in IGFBP-2 knockdown and negative control Bxpc-3 and CFPAC cells. (B) The expression of IGFBP-2, PTCH1 and Gli1 was detected by immunohistochemistry in orthotopic tumor tissues (Original mignification, 20×). (C) The expression of PTCH1 after treatment with PTCH1 siRNA. (D) Western blot assays were underwent in groups of the si-IGFBP2, si-PTCH1 and co-transfected with both si-IGFBP2 and si-PTCH1. The expressions of PTCH1, E-cadherin, vimentin and N-cadherin were detected. (E) Immunofluorescence staining showed the expression of E-cadherin and vimentin si-IGFBP2, si-PTCH1 and co-transfected with both si-IGFBP2 and si-PTCH1 (Original mignification, 20×). Data was presented as the means±SD of three independent experiments. ** compared with control, P<0.01. *** compared with control, P<0.001. ns, not significant.