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. 2017 Jun 28;8(37):61901–61916. doi: 10.18632/oncotarget.18717

Figure 3. Overexpression of astrocyte-elevated gene-1 induced thymidylate synthase expression and elevated resistance to pemetrexed.

Figure 3

Figure 3

Figure 3

Figure 3

(A) MTS assays demonstrated that astrocyte-elevated gene-1 (AEG-1) overexpressing PA11 and PA14 cell lines were more resistant to pemetrexed action than the primary PC-9 cell line. *p < 0.05. Transfection of cells with thymidylate synthase (TS) siRNA led to partial restoration of pemetrexed inhibitory action on cell viability in AEG-1 overexpressing cell lines PA11 and PA14. (B) Flow cytometry dot plots and quantitative analysis showed increased apoptosis rate in PA11 and PA14 cell lines after TS knockdown. *p < 0.05. (C) Western blotting and RT-PCR demonstrated that overexpression of AEG-1 in cell lines PA8, PA11, PA13, and PA14 led to relatively higher levels of TS expression. *: compare with PC-9, p < 0.05. (D) Decreased TS protein and mRNA levels following transfection of AEG-1-overexpressing cell lines PA11 and PA14 with TS siRNA were detected with western blotting and RT-PCR, respectively. *: compare with PA11 and PA14, p < 0.05, respectively.