Figure 4. CTCF expression is upregulated in metastatic lesions of ovarian cancer.

(A) CTCF expression was evaluated in metastatic (M) and primary lesions (P) from 12 ovarian cancer patients by Western blot analysis. Histone 3 was used as the reference protein. (B) The gray value of CTCF expression in (A) was calculated and analyzed. (C) IHC analysis was used to evaluate CTCF expression in metastatic and primary lesions from 20 ovarian cancer patients. Representative images are shown (scale bar, 50 μm). (D) CTCF IHC analysis results in (C) were scored and analyzed by paired t-test. Representative data from 3 independent experiments are shown. ^**P<0.01, ^***P<0.001.