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. 2017 Sep 12;6:e27421. doi: 10.7554/eLife.27421

Figure 3. Expression and activity of MIR165/166 is localized to the periphery of the shoot meristem.

Figure 3.

(A) Expression of MIR166Ap::GFPER (green), PIN1-CFP (blue) and REV-2 × YPet (red) in the vegetative meristem (VM) at 3.5 DAS. (B) Longitudinal section of meristem shown in (A). (C) Expression of MIR166Ap::GFPER (green), PIN1-CFP (blue) and REV-2 × YPet (red) in the inflorescence meristem (IM). White arrow head marks the reestablishment of MIR166Ap::GFPER expression around the meristem after organ emergence. (D to F) Expression of REV-2 × YPet (red) alone (D), a MIR165/166 biosensor driven by the UBQ10 promoter (green) alone (E) and both combined in the same IM (F). (G–I) Corresponding control for (D to F) where the MIR165/166 biosensor has been rendered insensitive to MIRNA activity. Bars represent 20 μm.