Figure 3.

Cell viability (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)) and cell proliferation (bromodeoxyuridine (BrdU)) assay. (A) Cell viability of LDC-3 treated HaCaT cells for 24 h tested by an MTT assay. The values for LDC-3 treated cells were calculated as the percental quotient of the LDC-3 value and the DMSO value; (B) Relative proliferation rate of untreated HaCaT cells and cells treated for 12 h with 5 µM, 50 µM and 100 µM LDC-3. Mitotic nuclei were identified by incorporation of BrdU, which were immunocytochemically detected by horseradish peroxidase (HRP)-linked antibody. The transformation of the tetramethylbenzidine (TMB) substrate by the bound HRP was measured by reading the absorbance at 450 nm. No statistical differences could be revealed (p > 0.05). Dunnett’s multiple comparisons test.