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. 2017 May 9;8(40):66975–66986. doi: 10.18632/oncotarget.17756

Figure 4. DNMT1 suppresses miR-138 expression by direct binding to the promoter region.

Figure 4

(A) Nuclear proteins were extracted from wild type 786O cells and interaction of DAB2IP with DNMTs or HDAC2 were validated by IP. (B) Co-localization of DAB2IP and DNMT1 in the nuclei of 786O cell lines was determined by immunofluorescent staining. Scale bar = 25 μm. (C) DNMT1 was knock-downed in 786O KD and 769P cells, and miR-138 expression was measured by qRT-PCR. (D) The status of DNMT1 (left panel) or MBD (right panel) binding to the miR-138 gene promoter region in 786O Con and KD were evaluated by ChIP assay. (E) 786O KD cells were treated with 5-Aza (5 μM) for 72 h, and the effect of 5-Aza treatment on DNMT1 or MBD binding to the miR-138 promoter region were determined by ChIP assay.