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. 2017 May 2;8(40):67017–67028. doi: 10.18632/oncotarget.17549

Figure 2. Chromene analog SP-6-27 disrupts the microtubular dynamics and inhibits migration in ovarian cancer cells.

Figure 2

Ovarian cancer cells (A2780) were treated with control (DMSO, vehicle) or SP-6-27 (0.5μM) for 24 h and stained with DAPI (blue) and an (A) anti–α-tubulin or (B) anti-β-tubulin antibody (green). Images were captured using an Olympus FluoView confocal microscope. Representative confocal micrographs (original magnification: 80X) are shown. (C) Effect of SP-6-27 on OVCA cell migration. In vitro wound healing assay was performed using A2780 cells cultured in 6 well plates. Confluent cultures were scratched with a 1 mL pipette tip as described in the Methods section. Representative phase-contrast images of cells migrating into the wounded area in SP-6-27 treated and control wells (0, 24 and 48 h) are shown here. W: wound space, WE: wound edge (magnification- 4X, scale bar-200 μm).