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. 2017 Jun 5;8(40):67218–67226. doi: 10.18632/oncotarget.18365

Figure 2. The effect of hernandezine on LPS-induced TNFα production in macrophage cells.

Figure 2

Human macrophage cells, U937 line (A and B) and THP-1 line (C and D), were treated with LPS (100 ng/mL) or plus hernandezine (1-30 μM), cells were further cultured in the conditional medium for indicated time, TNFα mRNA expression (A and C) and TNFα protein content (in conditional medium, B and D) were tested by qRT-PCR assay and ELISA assay, respectively. *p<0.05 vs. “C”. # p<0.05 vs. LPS only treatment. Experiments in this figure were repeated three times, and similar results were obtained.