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. 2017 Jun 5;8(40):67218–67226. doi: 10.18632/oncotarget.18365

Figure 3. Activation of AMPK is required for hernandezine-induced anti-LPS response.

Figure 3

U937 cells were treated with hernandezine (1-30 μM) for 2 hours, expression of listed proteins was tested by Western blotting assay, and blot data of three sets of repeat were quantified (A) U937 cells were infected with lentiviral AMPKα shRNA (“shAMPKα-No.1”/“shAMPKα-No.2”) or scramble control shRNA (“sh-C”), and stable cells were established; cells were treated with hernandezine (30 μM) for 2 hours, expression of listed proteins was tested by Western blotting assay (blot data of three sets of repeat were quantified (B)); cells were also treated with LPS (100 ng/mL), TNFα mRNA expression (C) and protein content (in conditional medium, (D)) were tested. “Ctrl” stands for un-infected cells. *p<0.05 vs. “C” (A). # p<0.05 vs. “sh-C” group (B). *p<0.05 vs. LPS only group (C and D). # p<0.05 vs. hernandezine of “sh-C” group (C and D). Experiments in this figure were repeated three times, and similar results were obtained.