Figure 3. Analysis of the subcellular localization of GFP-HO-1 in the positively characterized stable K562 cell lines 1-6 after 48 h hypoxia (1 % O2) by confocal laser scanning microscopy.
Under normoxia GFP-HO-1 is localized at the ER membrane. The nucleus is free from GFP-HO-1. After 48 h incubation under hypoxia GFP-HO-1 translocated to cytosol and nucleus. Figure shows representative data from five pictures per sample out of three independent experiments on a CLSM at 37°C. Bar represents 20 μm.