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. 2017 Sep 13;8(3):100. doi: 10.3390/insects8030100

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Substrate specificity and the effects of the reducing agents on the lipase activity. The posterior midgut homogenate (crude extract) of R. palmarum was incubated with the p-Nitrophenyl esters with different chain lengths (A). The effects of reducing agents (B) were determined by incubation with different concentrations of reduced glutathione (GSH) (open circles), dithiothreitol (DTT) (open triangles), and oxidized glutathione (GSSG) (filled circles). Lipase activity was measured using C16 pNP-palmitate as substrate. The error bars represent the SD for the three determinations (* p < 0.05).