Retraction

Medical Sciences. In the article “Potent inhibition of human immunodeficiency virus type 1 replication by an intracellular anti-Rev single-chain antibody” by Lingxun Duan, Omar Bagasra, Mark A. Laughlin, Joseph W. Oakes, and Roger J. Pomerantz, which appeared in number 11, May 24, 1994, of Proc. Natl. Acad. Sci. USA (91, 5075–5079), the undersigned authors wish to note the following: “The sequence of the heavy chain of the D8 anti-Rev single chain variable fragment (SFv) has been reanalyzed and found to be not what was reported in the article. It is likely that the coding DNA was derived from the fusion partner cell line used to make the original D8 hybridoma, and not from the heavy chain gene expressed by the B cell precursor to this hybridoma. There is a deletion in the framework region 3 (FR3) leading to a framehsift in CDR3 and downstream regions of the heavy chain gene. Individual nucleotide differences make this sequence very close to that described [Thammana, P. (1994) Mol. Immunol. 31, 77–78]. The initial 12 amino acids in the D8SFv represent a portion of V_k leader sequence. Minimal binding data for the D8SFv was available for reevaluation, including only a single ELISA for the D8SFv to recombinant Rev and a single binding study to the activation domain peptide of Rev. Although we did not state this in the original article, we did not directly compare binding of the SFv to the original D8 monoclonal antibody, and we did not fully characterize all the original monoclonal antibody’s binding parameters. We regret any difficulties these inaccuracies in the original publication may have caused.”