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. 2017 Aug 25;9(9):937. doi: 10.3390/nu9090937

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Measurement of GPR120, ERK, Raf and IKKβ. (A) Effect of EPA on the protein expression of GPR120 in RAW264.7 cells; (B) Effect of EPA on the protein expression of ERK1/2 in RAW264.7 cells; (C) Effect of EPA on the protein expression of Raf and IKKβ in RAW264.7 cells. Cells were treated with DMSO (equivalent volume 3.0 μM EPA, as control) or various concentrations (1.2, 1.8, 2.4 and 3.0 μmol) of EPA for 24 h. The expression of protein was analyzed by Western blot. The β-actin was used as an equal loading control (means ± SD, * p < 0.05 and ** p < 0.01 compared with the control). Each bar is representative of 6 independent experiments, and data were analyzed by ANOVA and Duncan’s multiple range tests.

Measurement of GPR120, ERK, Raf and IKKβ. (A) Effect of EPA on the protein expression of GPR120 in RAW264.7 cells; (B) Effect of EPA on the protein expression of ERK1/2 in RAW264.7 cells; (C) Effect of EPA on the protein expression of Raf and IKKβ in RAW264.7 cells. Cells were treated with DMSO (equivalent volume 3.0 μM EPA, as control) or various concentrations (1.2, 1.8, 2.4 and 3.0 μmol) of EPA for 24 h. The expression of protein was analyzed by Western blot. The β-actin was used as an equal loading control (means ± SD, * p < 0.05 and ** p < 0.01 compared with the control). Each bar is representative of 6 independent experiments, and data were analyzed by ANOVA and Duncan’s multiple range tests.