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. 2017 Sep 11;9(9):1001. doi: 10.3390/nu9091001

Figure 5.

Figure 5

Effects of 5-oxo-ETE on Nrf2 activation in HUVECs. Nuclear translocation of Nrf2 was detected by immunostaining. The cells were treated for 3 h with: 0.5% MeOH (vehicle control) (A); and 5 μM 5-oxo-ETE (B); and the pretreatment with 400 μM α-tocopherol for 1 h was performed before treatment of 5-oxo-ETE (C). The intensity of the green fluorescence signal in the nucleus was quantified using Image J (D); Data are expressed as the mean ± SD (n = 8) Significant differences among the groups are indicated with different letters (one-way ANOVA followed by a post hoc Tukey’s test, p < 0.05). Gene expression of HMOX1 in the cells treated with 1, 5, and 10 μM 5-oxo-ETE for 6 h was analyzed (E). Data are expressed as mean ± SD (n = 3). Significant differences among the groups are indicated with different letters (one-way ANOVA followed by a post hoc Tukey’s test, p < 0.05). ROS generation in HUVECs was detected by CellROX Green (F). HUVECs were treated with 400 μM α-tocopherol for 1 h, and subsequently the cells were incubated for 1 h after the addition of 5 μM 5-oxo-ETE. Data are expressed as the mean ± SD (n = 4).