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. 2017 Sep 25;6(9):e381. doi: 10.1038/oncsis.2017.80

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Copyright © 2017 The Author(s)

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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PRAS40 associates with P65. (a) Plasmids expressing GST-PRAS40, GST-only, Venus-p65 or Venus only were transfected as indicated. Glutathione beads were used to precipitate GST-tagged proteins. Eluates and input lysates were analyzed by western blotting. (b) Endogenous PRAS40 was co-immunoprecipitated with p65 in melanoma A375 and lung cancer A549 cells.