(
A) Overlap between Zld ChIP-seq peaks from
Harrison et al. (2011) and Bcd ChIP-seq peaks defined in this study. A total of 732 of Bcd ChIP peaks (71.3%) overlap with Zld peaks. (
B) Odds ratios and p-values calculated from Fisher's exact tests for enrichment of each Bcd peak class in peaks that are also bound by Zld. In contrast to Zld dependence as defined by reduced chromatin accessibility in
zld– embryos, Concentration Sensitive I and II classes show a greater enrichment for Zld binding than the Concentration Sensitive III and Insensitive classes. (
C) Odds ratios and p-values calculated from Fisher's exact tests for enrichment of each Bcd peak class in Zld dependent peaks, separated into those bound and not bound by Zld. Concentration insensitive peaks that are bound by Zld are overrepresented in Zld dependent peaks, while Concentration Sensitive I peaks are underrepresented. In Bcd peaks that are not bound by Zld, no sensitivity class is significantly enriched for Zld dependence. (
D) Odds ratios and p-values calculated from Fisher's exact tests for enrichment of each Bcd peak class in Bcd dependent peaks, separated into those bound and not bound by Zld. Concentration Sensitive I and II peaks that are also bound by Zld are overrepresented in Bcd dependent peaks, while Concentration Sensitive III and Insensitive peaks are underrepresented. In Bcd peaks that are not bound by Zld, no sensitivity class is significantly enriched in for Bcd dependence.