Table 1.

Single Nucleotide Polymorphisms (SNPs) and other changes found after re-sequencing.

Gene	Description	Position	630 12	630 22	630	C	F	630Δerm	C	F	630E	C	F	AA
630E
CD630_07610	Putative ATP-dependent RNA helicase	933139	G	—	—			—			T	199	100	Asp136Tyr
CD630_14040	Putative oligopeptide transporter	1626977	A	—	—			—			G	187	100	Glu536Gly
CD630_20270	N-carbamoyl-L-amino acid hydrolase	2339506	G	—	—			—			A	73	100	Gly373Glu
CD630_26670	PTSG-BC	3079815	A	—	—			—			C	165	100	*524Glu
CD630_26270	Hypothetical protein	3034953	C	A	A			A			—	156	100	Gly68Cys
CD630_33790	conjugative transposon protein	3951559	C	—	—			—			A	307	100	Glu63Asp
CD630_12740	topA	1480649	C	—	—			—			T	146	100	Gln386*
CD630_29430	Putative phage replication protein	3422569	T	—	—			—			C	195	100	Asn210Asp
IG	Intergenic region	309208	—	—	—			—			INV	129	41
IG	Intergenic region	3528736	G	—	—			—			T	94	100
630Δerm
CD630_19070	eutG	2209236	G	—	—			A	127	97				Gly252Glu
CD630_35650	GntR family transcriptional regulator	4166495	G	—	—			A	182	100	—			Ala91Val
IG	Intergenic region	2937176	C	—	—			A	173	100	—
IG	Intergenic region	3005866	T	—	—			G	156	100	—
IG	Intergenic region	3591103	G	—	—			A	211	100	—
CD630_12140	spo0A	1413057	—	—	—			AGAATGT-AGGAAA-TATAG	112	40	—			Insertion
CD630_08260	Ferric uptake regulator	1000995	A	—	—	100	100	G	153	100	—	170	98	Thr41Ala
630
CD630_32450	prdR	3797112	C	—	T	117	100	—			—			Glu261Lys
CD630_02050	Transcription antiterminator, PTS operon regulator	268934	G	—	T	123	97	—			—			Gly165Cys
630 and 630Δerm
CD630_2667	PTSG-BC	3080703	C	—	T	75	100	T	141	100	—			Val228Ile
IG	Intergenic region	2203033	A	—	T	105	100	T	174	99
IG	Intergenic region	4007463			C	10	100	C	62	100
630 and 630Δerm and 630E
CD630_11900	acyl-CoA N-acyltransferase	1391850	T	—	C	118	100	C	133	100	C	143	100	Phe133Leu
CD630_13880	pseudo	1607453	INS 1	—	T	119	94	T	175	94	T	124	88	Thr16fs
Mistake in original sequence
CD630_17670	gapB	2044514	C	G	G			G			G
CD630_31561	pseudo	3686535	INS 1	A	A			A			A

The table shows the Single Nucleotide Polymorphism (SNP) changes in 630Δerm and 630E compared to the reference 630¹² and also the new annotation by Riedel et al (The column ‘Gene’ represents the gene (or intergenic region (IG)) in which the change occurs, the column ‘position’ indicates the exact nucleotide position of the change.).²² It also contains SNP frequency (F) and genomic coverage (C) as well as the resultant amino acid change (AA). No change from the original 630 annotation¹² is represented by a dash (–).