Table 1.
Antibodies, sources and working dilutions.
| Antigen | Working dilution | Host | Clone or catalog number | Source |
|---|---|---|---|---|
| pS6 | 1:750 | rabbit | #2215; Ser240/244 | Cell Signaling |
| Sox2 | 1:1000 | goat | Y-17 | Santa Cruz Immunochemicals |
| Egr1 | 1:1000 | goat | AF2818 | R&D Systems |
| BrdU | 1:200 | rat | OBT00030S | AbDSerotec Raleigh, NC |
| Phosphorylated-p38 MAPK | 1:400 | rabbit | 12F8 | Cell Signaling Technologies |
| TopAP | 1:100 | mouse | 2M6 | Dr. P. Linser University of Florida |
| Sox9 | 1:2000 | mouse | AB5535 | Chemicon |
| Pax6 | 1:50 | mouse | PAX6 | DSHB |
| Pax6 | 1:1000 | rabbit | PRB-278P | Covance |
| Klf4 | 1:50 | rabbit | ARP38430 | Aviva Systems Biology |
| pERK1/2 | 1:200 | rabbit | 137F5 | Cell Signaling Technologies |
| cFos | 1:400 | rabbit | K-25 | Santa Cruz Immunochemicals |
| pSmad1/5/9 | 1:200 | rabbit | D5B10 | Cell Signaling Technologies |
| Smad2 | 1:300 | rabbit | D43B4 | Cell Signaling Technologies |
| Glutamine Synthetase | 1:2000 | mouse | ab125724 | Abcam |
| CD45 | 1:200 | Mouse | HIS-C7 | Cedi Diagnostic |
| Nkx2.2 | 1:80 | Mouse | 74.5A5 | DSHB |