(Developmental Cell 26, 393–404; August 26, 2013)
Following the online publication of this paper, the authors noticed that the control loading panel for total MET levels in the nontreated A549-GFP cells in Figure 5A was incorrectly inserted. The mistake has been amended online and the correct western blot panel has been inserted in the final version of Figure 5, shown below. The authors apologize for any possible confusion this error might have caused.
Figure 5.
TNS4-MET Interaction Attenuates MET Internalization and Lysosomal Targeting
(A) Endocytosis rate of biotinylated cell-surface MET in A549-TNS4_WT-GFP and control A549-GFP cells ± HGF (30 ng/ml) (mean ± SEM band intensity normalized to end point [20 min]; n = 3). Statistical differences at each time point between GFP- and TNS4_WT-GFP-overexpressing cells were analyzed by Student’s t test.
(B) FACS analysis of cell-surface MET endocytosis rate in A549-GFP, A549-TNS4_WT-GFP, or A549-TNS4_R474A-GFP cells ± HGF (30 ng/ml, 30 min) (mean ± SEM fluorescence intensity; n = 3).
(C) Total MET levels in control-, TNS4-, and GGA3-silenced A549 cells treated with DMSO or HGF (30 ng/ml, 1 hr) (mean ± SEM band intensity normalized to control-silenced cells; n = 3). Statistical significance was analyzed between control-silenced cells and the other conditions.
(D) Endocytosis rate of biotinylated cell-surface β1-integrin was measured as described in (A).
(E) MET and active β1-integrin coendocytosis upon HGF induction (30 ng/ml) in A549 cells labeled with cell-surface-bound MET (antibody directed against extracellular domain) and β1-integrin (12G10) antibodies. Scale bar, 10 μm.
(F) A549 cells cotransfected with TNS4 _WT-GFP or TNS4_R474A-GFP mutant and Lamp1-RFP were stained for MET (antibody directed against extracellular domain) after HGF (30 ng/ml) induction. r: Pearson’s correlation coefficient between Lamp1-RFP and MET staining in cells (mean ± SEM; n = 7 cells per condition). Scale bar, 10 μm. ∗p < 0.05, ∗∗p < 0.005, ∗∗∗p < 0.0005.