Figure 9.

Rapamycin does not increase induced regulatory T (iTreg) cell differentiation in the absence of Ikaros but restoring Foxo1 activity does. (a) GFP expression levels in iTreg cell differentiation cultures performed with wild‐type or Ikaros null (IK ^−/−) splenocytes from Foxp3/GFP reporter mice either with or without 25 nm rapamycin. Data are representative of two independent experiments. (b) Purified splenic CD4 T cells from Ikaros null (IK−/−) and wild‐type mice were plated under iTreg cell differentiation conditions and transduced with retroviruses prepared from the MSCV Thy‐1.1 construct (EV) or the MSCV Foxo1‐A3 Thy‐1.1 construct (Foxo1‐A3). Data shown are intracellular Foxp3 staining in successfully transduced Thy‐1.1⁺ cells 4 days after transduction. Data are representative of two independent experiments.