Table 2. The effect of DSF:Cu and ionising radiation on the progression of synchronised cells through G2.
| Control | DSF:Cu | 5 Gy | DSF:Cu + 5 Gy | |
|---|---|---|---|---|
| SK-N-BE(2c) | ||||
| G1 | 30.5 ± 0.70 | 17.2 ± 0.27*** | 16.0 ± 0.79*** | 17.2 ± 0.12*** |
| S | 18.8 ± 0.31 | 28.4 ± 0.48*** | 17.0 ± 1.10 | 28.3 ± 0.76*** |
| G2 | 33.7 ± 0.40 | 39.2 ± 0.62 | 42.4 ± 3.15* | 37.3 ± 1.09 |
| UVW | ||||
| G1 | 86.5 ± 0.18 | 4.6 ± 0.70*** | 13.2 ± 1.49*** | 5.1 ± 0.12*** |
| S | 2.4 ± 0.08 | 1.2 ± 0.06 | 1.6 ± 0.06 | 1.8 ± 0.53 |
| G2 | 9.6 ± 0.33 | 93.8 ± 0.79*** | 84.3 ± 1.62*** | 92.8 ± 1.65*** |
The percentages of synchronised SK-N-BE(2c) and UVW cells in G1, S and G2 were determined by FACS analysis of propidium-iodide-stained cells 6 h after irradiation at the S-G2 border and treatment with 0.3 μM DSF:Cu. Data are means ± SEM, n=3. One-way ANOVA with Bonferroni correction was used to compare the means of the test groups with those of the untreated control (*). One symbol: P<0.05; two symbols: P<0.01; three symbols: P<0.001.