One data point is inadvertently omitted from the upper left graph in Fig 5. The authors have provided the corrected version here.
Several data points were inadvertently omitted from the data set used for analysis (S1 Dataset). These data points should be included, and Tables 2, 3, 4 and 5 should be changed due to this addition. This does not affect the conclusions of the paper.
Table 2. R2 values describing the fit of the translocation data as a function of days post planting (DPP) for clothianidin as estimated by the exponential decay function: C = C0e−kt.
Region | Treatment | 2014 | 2015 |
---|---|---|---|
Root | Naked | 0.101 | 0.306 |
Fung | 0.499 | 0.618 | |
Low | 0.461 | 0.344 | |
High | 0.421 | 0.437 | |
Seed | Naked | 0.272 | 0.210 |
Fung | 0.287 | 0.400 | |
Low | 0.494 | 0.041 | |
High | 0.244 | 0.027 | |
Shoot | Naked | 0.351 | 0.347 |
Fung | 0.523 | 0.681 | |
Low | 0.585 | 0.731 | |
High | 0.541 | 0.86 |
*P < 0.05 **P < 0.01 ***P < 0.001
Table 3. F-values and estimated degrees of freedom (df) for the multivariate repeated-measures ANOVA model describing in-plant concentrations of clothianidin over the sampling period in 2014 and 2015 for the three plant regions (Root, Seed, Shoot).
Region | 2014 Factor | df | F-value | 2015 Factor | df | F-value |
---|---|---|---|---|---|---|
Root | Time | 7,3 | 418.22*** | Time | 8,1 | 391.84* |
Treatment*Time | 21,9.17 | 4.64* | Treatment*Time | 24,3.50 | 4.43 | |
Seed | Time | 6,4 | 314.37*** | Time | 6,3 | 14.05* |
Treatment*Time | 18,11.80 | 1.15 | Treatment*Time | 18,8.97 | 2.42 | |
Shoot | Time | 7,3 | 181.26*** | Time | 8,1 | 328.26* |
Treatment*Time | 21,9.17 | 2.27 | Treatment*Time | 24,3.50 | 1.25 |
*P < 0.05
**P < 0.01
***P < 0.001
Table 4. Univariate F-values and degrees of freedom (df) generated following the multivariate repeated-measures ANOVA model describing influence of initial clothianidin seed treatment (Treat) on in-plant concentrations of clothianidin over the course of multiple days post planting (DPP) in 2014 and 2015 and three plant regions (Root, Seed, Shoot).
2014 | F-value | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Region | Factor | df | 6 DPP | 8 DPP | 10 DPP | 13 DPP | 15 DPP | 17 DPP | 20 DPP | 34 DPP | |
Root | Treat | 3,9 | 142.15*** | 79.27*** | 32.59*** | 67.51*** | 177.66*** | 21.51*** | 17.20*** | 3.69 | |
Seed | Treat | 3,9 | 71.67*** | 112*** | 63.74*** | 84.58*** | 143.84*** | 52.19*** | 24.07*** | ||
Shoot | Treat | 3,9 | 40.78*** | 121.11*** | 116.15*** | 64.44*** | 108.55*** | 91.78*** | 37.15*** | 1.65 | |
2015 | |||||||||||
Region | Factor | df | 5 DPP | 7 DPP | 9 DPP | 12 DPP | 14 DPP | 16 DPP | 19 DPP | 47 DPP | 61 DPP |
Root | Treat | 3,8 | 52.89*** | 24.97*** | 321.89*** | 50.81*** | 159.45*** | 71.75*** | 62.14*** | 2.34 | 2.77 |
Seed | Treat | 3,8 | 424.34*** | 369.71*** | 715.73*** | 128.60*** | 82.35*** | 93.66*** | 44.11*** | ||
Shoot | Treat | 3,8 | 429.43*** | 79.38*** | 229.14*** | 37.17*** | 101.01*** | 93.13*** | 87.86*** | 1.87 | 0.19 |
*P < 0.05
**P < 0.01
***P < 0.001
Table 5. F-values of a priori contrasts comparing untreated maize seed (Fungicide + Naked) to 0.25 mg clothianidin/kernel (Low) and 1.25 mg clothianidin/kernel (High) at various days post planting (DPP) for three different plant regions (Root, Seed, Shoot) in 2014 and 2015.
2014 | 2015 | ||||
---|---|---|---|---|---|
Region | Contrast | DPP | F-value | DPP | F-value |
Root | Fungicide+Naked vs Low | 15 | F1,9 = 38.86*** | 19 | F1,8 = 57.11*** |
17 | F1,9 = 1.89 | 47 | F1,8 = 5.11 | ||
20 | F1,9 = 0.66 | 61 | F1,8 = 2.81 | ||
Fungicide+Naked vs High | 17 | F1,9 = 57.59*** | 19 | F1,8 = 180.27*** | |
20 | F1,9 = 47.08*** | 47 | F1,8 = 4.09 | ||
34 | F1,9 = 10.13* | 61 | F1,8 = 1.41 | ||
Seed | Fungicide+Naked vs Low | 17 | F1,9 = 18.98** | 19 | F1,8 = 53.89*** |
20 | F1,9 = 3.97 | ||||
Fungicide+Naked vs High | 17 | F1,9 = 155.55*** | 19 | F1,8 = 110.31*** | |
20 | F1,9 = 69.20*** | ||||
Shoot | Fungicide+Naked vs Low | 17 | F1,9 = 26.69*** | 19 | F1,8 = 89.05*** |
20 | F1,9 = 8.17* | 47 | F1,8 = 0.38 | ||
34 | F1,9 = 0.56 | ||||
Fungicide+Naked vs High | 20 | F1,9 = 107.03*** | 19 | F1,8 = 242.76*** | |
34 | F1,9 = 4.47 | 47 | F1,8 = 4.77 |
*P < 0.05
**P < 0.01
***P < 0.001
Please see the complete, correct S1 Dataset below.
Supporting information
Reference
- 1.Alford A, Krupke CH (2017) Translocation of the neonicotinoid seed treatment clothianidin in maize. PLoS ONE 12(3): e0173836 https://doi.org/10.1371/journal.pone.0173836 [DOI] [PMC free article] [PubMed] [Google Scholar]
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