Figure 3.

Primary bone marrow stromal cells (BMSCs) were treated with vehicle (A, C) or TriC (B, D) during the first 24 hours of osteogenic differentiation; treatments were then removed and cells were allowed to differentiate an additional 7 days, and stained for alkaline phosphatase (ALP) and Von Kossa. To confirm TriC's ability to blunt lipid droplet formation, BMSCs were stained with BODIPY493/503 and Hoechst immediately following the initial 24 hour treatment period of vehicle (E) or TriC (F). Additionally, vehicle (G) or TriC (H) treated BMSCs were stained with hematoxylin to confirm cell viability following the initial 24 hours of treatment.