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Growth profile of E. coli DH5α harboring the plasmid pHBSR1-RM-pre-miR-29b cultivated in TB medium at 37°C and 250 rpm (red line/primary scale). Time-course analysis of intracellular pre-miR-29b production in E. coli DH5α cultures (black line/secondary scale) measured by quantitative RT-PCR using a specific probe and as described previously9 Error bars indicate standard deviations calculated from 3 independent samples.
