Figure 2.

Growth and E-lysis of E. coli C41 carrying plasmids for expression of INP and BG production. E. coli C41 (pBINP, pGLMivb) (quad) and E. coli C41 (pBINP, pGLysivb) (triangle) cultures were grown in LBv supplemented with 0.2% arabinose to induce INP expression at 23°C from plasmid pBINP. At time point zero (illustrated by vertical sketched line) E-mediated lysis was induced from pGLysivb by temperature shift from 23°C to 42°C and from pGLMivb by addition of 0.5 mM IPTG. Open symbols indicate OD₆₀₀ values of growth and lysis phase. During E-lysis colony forming units (closed symbols) were determined to calculate lysis efficiency. After 120 min (E. coli C41 [pBINP, pGLysivb]) and 150 min (E. coli C41 [pBINP, pGLMivb]) BG production was completed. Data were obtained from 3 independent experiments. Error bars indicate standard errors.