Figure 4. Gene expression analysis of canine glioma stem cells.

(A) A three-dimensional representation of a principle component analysis of expression microarray data derived from untreated J3T adherent cells (0 Gy), irradiated adherent cells (5Gy), untreated spheres (0 Gy) and irradiated spheres (5 Gy). (B) Hierarchical clustering analysis of the expression data (cut off p-value of 0.005). Expression values are represented by colours: blue squares represent low-expressed genes, red squares represent high-expressed genes. (C) Biological process analysis of differentially expressed genes in J3T spheres compared to adherent cells (FDR = 0.005). (D) Validation of microarray with qRT-PCR.