Figure 3. The effect of silibinin on radiation-induced EMT in IEC6 cells.

A. Immunofluorescence of α-SMA and counterstaining with DAPI. IEC6 cells were treated with 20 μm silibinin 3 h prior to 10 Gy of IR and incubated for 72 h. Scale bar = 50 μm. B. Western blotting for N-cadherin, E-cadherin and α-SMA in irradiated IEC6 cells and C. quantification. The data are shown as the mean±SD; n = 3, *p < 0.05 and **p < 0.01.