Fig. 2.

Characterization of primary mixed murine retinal cultures enriched in RGCs. Immunostaining of primary murine cultures reveals a high concentration of cells labelled with RGC specific markers [A] Brn3a, [B] γ-synuclein, [C] RBPMS [D] Thy-1. Hoechst nuclear staining (blue) with immunostaining (FITC/TRITC). Scale bar = 20 μm, × 10 magnification. [E] Results were confirmed with reverse-transcriptase PCR using primers against (1) Thy-1, (2) Brn3a and (3) γ-synuclein. Band sizes were confirmed by comparison to appropriate molecular weight ladder; 200 bp (L200) or 100 bp (L100). No bands were detected in primary cell-free controls (data not shown) ruling out primer-dimers. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)