Figure 3.

Two-photon (λ_ex = 730 nm) induced images and cell death of HeLa cells incubated with PtGdL at different dose concentrations (1, 2, 5, 10 μM) for 24 h. (A) Without irradiation, there is no blue emission; (B) after 30 min excitation, blue emission from released GdL was observed; (C) Merged images of (B) and bright field of (A) shows significant cell death and blue emission intensity from the cells treated with PtGdL and 730 nm laser (Remark : IC₅₀ of PtGdL in HeLa cells is 23.6 ± 0.8 μM).