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. Author manuscript; available in PMC: 2018 Oct 27.
Published in final edited form as: Vaccine. 2017 Sep 28;35(45):6112–6121. doi: 10.1016/j.vaccine.2017.09.058

Figure 1. Gene expression and replication of WT H3N2 and antigenically-matched live-attenuated viruses in differentiated hNEC cultures.

Figure 1

Differentiated human nasal epithelial cell cultures were infected at a multiplicity of infection (MOI) of 1 and incubated at 32°C. (A) Measurement of infectious virus particle production in apical supernatant of infected hNEC cells. (B) Expression of cell-associated viral M2 mRNA expression was determined by quantitative RT-PCR. * indicates p>0.05. Three to four infected wells were averaged for the data points shown and each data point represents one donor with 5–7 donors were used for each time point.