FIGURE 5.

(A) RT-qPCR analysis of BF and PF CN cells that constitutively express V5-tagged WT, G163R, or G163V mutant versions of KREPB4, and in which the tet-regulatable WT KREPB4 allele was expressed (E) or repressed (R) for 2 and 4 d, respectively. The abundances of transcripts from KREPB4 alleles (B4 reg = tet-regulatable WT allele; B4 EE = exclusively expressed V5-tagged WT or mutant allele; B4 ORF = B4 open reading frame), and never-edited (COI and ND4), pre-edited, and edited mitochondrial mRNAs in repressed cells were calculated relative to cells expressing tet-regulatable WT KREPB4. For each target amplicon, relative abundance was determined using TERT as an internal control. Heat map shows the log₁₀-transformed relative abundances of RNAs from BF cells (left) and PF cells (right), as indicated by the scale bar (bottom). Data represent mean of three experiments. (B) Data from A, presented as means ± SEM, plotted on a log₁₀ scale. Asterisks indicate that the target amplicon was not detected in the repressed cells.