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. Author manuscript; available in PMC: 2017 Dec 19.
Published in final edited form as: Oncogene. 2017 Jun 19;36(42):5852–5860. doi: 10.1038/onc.2017.180

Figure 1. MKK3 interacts with proteins beyond p38.

Figure 1

A) Diagram showing new MKK3 binding partners and associated pathways. B) Validation of the MKK3 interaction with partners in a TR-FRET assay. The TR-FRET assay was performed for Venus-Flag-tagged MKK3 co-expressed in HEK293T cells with GST-fusions (light grey bars). Venus-Flag-MKK3/GST and GST-p38/Venus were included as negative controls (white bars), Venus-Flag-MKK3/GST-p38 served as a positive control (dark grey bar). The data was obtained in triplicate, bars represent averaged TR-FRET signals expressed as the FRET ratio (520nm/486nm × 104). C) The interactions of MKK3 with its partners were validated in a GST-pull down assay performed for GST-MKK3 co-expressed in HEK293T cells with Venus-Flag fusions. Venus-Flag-tagged p38 and Flag-Venus alone served as positive and negative controls for the assay.